Disposing of microbiological culture media

1.0       OBJECTIVE : To lay down a procedure for disposing of microbiological culture media.

2.0       RESPONSIBILTY

            Microbiologist /Q. C Executive

3.0       ACCOUNTABILITY

            Quality  Control  Manager

4.0             PROCEDURE

4.1           INTRODUCTION:

4.1.1  Bacteriological culture media contains all the nutritive sources which favors rapid growth of different types of Micro-organisms. Some selective media’s are used for cultivating specific type of Pathogenic   organisms. The  micro-organisms  grown  either   on enriched  or selective media’s if not disposed  or  destroyed  in proper  way can cause contamination of the   environment  which  in turns is very hazardous.

4.2           METHOD:

4.2.1     Collect all the used semi solid media’s in plastic bag containing about 50 ml of 2.5% Savlon solution and close the opening tightly with a tag or rubber-band and transfer  the it to the  incinerator.

4.2.2     For liquid media add 2.5% disinfectant (savlon), to the liquid media ,mix it well ,hold for half an hour and then discard them in a stream of running tap water.

4.2.3       The plates & test tubes are then thoroughly washed with soap water and sterilized by autoclaving.

4.3       Procedure for cleaning of glass-wares

4.3.1   INTRODUCTION

Proper cleaning of glassware’s used in microbiology laboratory is very important in microbiological work.  If the equipments used in microbiology are not cleaned properly, due to contamination, the microbiological results may get affected; also it can cause danger to the persons handling the equipment and risk of infection due to potential hazards of pathogenic cultures.

GMP  require  the  standard  procedure  for  glassware  cleaning  used  in  microbiological  work.  This  procedure  provides Guidelines  for  proper  cleaning  of  glassware  in  micro  section.            

4.3.2       Method For Cleaning

4.3.2.1Keep all the glassware’s used in microbiology in the cupboard of  the tables used by the Microbiologists to work.                                       

4.3.2.2Do not use these glass wares in chemical testing.                                                                        

4.3.2.3Rinse all the glass wares with tap water two to three times.                                                         

4.3.2.4Dip all the glass wares in 2% v/v solution (prepared in D.M water)of teepol or liquid  soap for at least 4 hours, for depyrogenation  dip it into  2.5% NaOH  solution.    

          4.3.2.5Remove the glass wares from  the solution.

4.3.2.6Rinse twice with D.M water followed by rinsing once with W.F.I              

4.4     ABBREVIATIONS

W.F.I= Water For Injection, NaOH= Sodium hydroxide, %=Percentage,  v/v =Volume by volume D.M water= De mineralized water

5.0       REASON FOR REVISION

Harmonization of format.

6.0       TRAINING:

Trainer --        Head – Quality Control

      Trainees--      Quality Control Chemists & Assistants 

Period    --     One day

7.0       DISTRIBUTION:

Certified Copy No.   1           :  Head of Department – Quality Control

Original Copy                        :  Head – QUALITY ASSURANCE.