1.0 OBJECTIVE
To lay down a procedure for operation and cleaning of autoclave
2.0 RESPONSIBILTY
Microbiologist./ Q.C Executive
3.0 ACCOUNTABILITY
Head - Quality Control
4.0 PROCEDURE
4.1 : PRE-START UP
4.1.1 : Ensure the cleanliness of the room and machine.
4.1.2 : Ensure that all utilities such as pure steam, water, power etc are available.
4.1.3 : Check that material are ready for auto clave and Load the autoclave as per load pattern
given in Annexure-1 along with the necessary temperature indicators.
4.1.4 : Ensure AHU of area is working.
4.1.5 : Inside door of the autoclave is closed properly.
4.2 : START UP
4.2.1 : Open pure steam valve from service floor.
4.2.2 : Open main valve of steam line .
4.2.3 : Open steam by-pass valve for condense out let.
4.2.4 : Close steam by pass valve after completely out let of condensate
4.2.5 : Open main valve on steam line connecting to chamber.
4.2.6 : Open the valve before trap on steam line.
4.2.7 : Close the door of the autoclave tightly.
4.2.8 : ON machine by pressing green color OFF/ON switch on loading side panel to start the
sterilization cycle.
4.2.9 : Press the F1 button of the PLC of autoclave after getting auto mode indicated by a red
indicator.
4.2.10 : Press F3 for cycle selection , select the cycle and press enter. selection of auto mode.
4.2.11 : Then press start button
4.2.12 : Close the outlet of jacket and chamber steam.
4.2.13 : When the PLC of autoclave shows sterilization cycle complete switch off the autoclave
and open the outlet of chamber and steam.
4.2.14 : After each day of working clean ( by moping) the autoclave with disinfectant solution.
4.3 : SHUT DOWN.
4.3.1 : At the end of cycle, Unload the sterilized material from sterile side
4.3.2 : Check the change in steam clox indicator. It should change as per Manufacturer instructions.
4.3.3 : Attach steam-clox in media preparation record and note down operation.
5.0 : Cleaning :
5.1 : Pre checks.
5.1.1 : Ensure that loading door is open and door to sterile side is closed.
5.1.2 : Ensure that autoclave panel electrical supply cut off.
5.1.3 : Ensure that no material is present inside the autoclave.
5.1.4 : Ensure that outer surface of autoclave is cool and is at room temperature.
5.1.5 : Ensure that all utility supply links to the autoclave are closed.
5.2 : Daily cleaning : At the end of shift.
5.2.1 : Clean the utility inlet holes with a nylon brush and them mop with lint free cloth.
5.2.2 : Mope the chamber, probes, loading trolley & outer surface of autoclave with lint free
cloth dipped in purified water.
5.2.3 : Mope the autoclave chamber and loading trolley with water for injection.
5.2.4 : Wipe the chamber, probes, utility pendants and outer surface of the autoclave with lint
free cloth.
6.0 : Precaution :
6.1 : Jacket pressure should not be less than 1.2 kg per cm square.
7.0 : Abbreviations:
AHU = Air Handling Unit
Programmable Logic Control (PLC)
8.0 : Annexure : Annexure – 1 attached
9.0 : Reference : Instrument Manual
ANNEXURE I
Details of the abbreviations used in Schematic represented.
A - Media-Probe ,B.I and C.I. inside the media
B,C,Q,R,S-Media ,
D-Dress -Probe ,B.I and C.I. inside the Dress ,
E-Pippet Carrier ,
F- Pippet –Probe,B.I and C.I. inside the Carrier ,
G-Petri-Plate Carrier ,
H-Petri-Plate -Probe ,B.I and C.I. inside the Carrier ,
I-Petri-Plate Carrier ,
J-Petri-Plate -Probe ,B.I and C.I. inside the Carrier,
K-Petri-Plate Carrier ,
L-Manifold -Probe ,B.I and C.I. inside Filter Holder ,
M-Manifold Filter Holder ,
N-Liquid Media -Probe ,B.I and C.I. inside media
O-S-S Perforated Box With Filter Assembly
P-S-S Box With -Probe ,B.I and C.I. inside the Assembly , Filter Assembly
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