1.0 OBJECTIVE : To lay down a procedure for operation of compound microscope.
2.0 RESPONSIBILTY
Microbiologist / Executive
3.0 ACCOUNTABILITY
Head – Quality Control
4.0 PROCEDURE
4.1 Keep the microscope on the clean and stable platform.
4.2 Switch on the main switch of microscope so that bulb will glow.
4.3 Adjust the low power objective, by rotating the revolving nose piece in position.
4.4 Adjust the focus of light with the help of condenser.
4.5 Now place the objective ( high power / oil immersion whichever is required) in position by moving the resolving nose piece.
4.6 Place the slides on the stage, set the objective at the closest position w.r.t slide then slowly lift the objective with coarse adjusting screw, while observing through the eye piece, till the field appears clearly ( if the oil immersion objective is in use, place on drop of immersion oil on the slide before placing it on the stage and then lower the objective just to touch the oil drop.
4.7 Make the field clearer with the help of fine adjustment and then observe the slide.
4.8 Slide can be moved in left, upper and lower direction to observe the complete smear area with help of slide stage screw and coarse adjustment.
4.9 After completion of observation, remove the slide, clean the objective lens and condenser with cotton soaked in xylene. Bring the 10x objective in the active position. Lower down the condenser and place low power objective in position.
4.10 Do not allow the oil to harden on the lens.
5.0 REASON FOR REVISION
Harmonization of format.
6.0 TRAINING:
Trainer -- Head – Quality Control
Trainees-- Quality Control Chemists & Assistants
Period -- One day
7.0 DISTRIBUTION:
Certified Copy No. 1 : Head of Department – Quality Control
Certified Copy No. 2 : For Display Near The Microscope
Original Copy : Head – QUALITY ASSURANCE
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